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andulex europeaus 1 lectin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories andulex europeaus 1 lectin
    Andulex Europeaus 1 Lectin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 519 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotinylated+uea+1+lectin/Biotinylated+Ulex+Europaeus+Agglutinin+I+(UEA+I)/10__1113_slash_jp286269-103-46-51
    Average 96 stars, based on 519 article reviews
    andulex europeaus 1 lectin - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Staining:

    Article Title: Nfkb2 variants reveal a p100-degradation threshold that defines autoimmune susceptibility
    Article Snippet: .. Surface staining of TECs was performed using the following antibodies for 20 min on ice: anti-mouse CD45 PerCP/Cy5.5 (catalog no. 103132; BioLegend), anti-mouse CD326 (EpCAM) APC/Cy7 (catalog no. 118218; BioLegend), and biotinylated UEA-1 lectin (catalog no. B-1065; Vector Laboratories). .. Intracellular staining with anti-mouse AIRE-A647 (clone 5H12; Walter and Eliza Hall Institute) was performed after fixation and permeabilization with the eBioscience Foxp3/Transcription Factor Staining Buffer Set (catalog no. 00–5523-00; Thermo Fisher Scientific).

    Article Title: Mevalonate metabolism–dependent protein geranylgeranylation regulates thymocyte egress
    Article Snippet: High-resolution images were acquired using a Marianis spinning disk confocal microscope (Intelligent Imaging Innovations) equipped with a 40× 1.3-NA objective; 405-nm, 488-nm, 561-nm, and 647-nm laser lines and a Prime 95B CMOS camera (Photometrics); and analyzed using Slidebook software (Intelligent Imaging Innovations). .. For immunofluorescence staining of thymus, freshly frozen thymi were cryosectioned and stained as outlined above using biotinylated UEA-1 lectin (Vector Labs). .. Sections were washed in PBS before incubation with AF568-conjugated streptavidin (ThermoFisher) and 10 nM DAPI.

    Immunofluorescence:

    Article Title: Mevalonate metabolism–dependent protein geranylgeranylation regulates thymocyte egress
    Article Snippet: High-resolution images were acquired using a Marianis spinning disk confocal microscope (Intelligent Imaging Innovations) equipped with a 40× 1.3-NA objective; 405-nm, 488-nm, 561-nm, and 647-nm laser lines and a Prime 95B CMOS camera (Photometrics); and analyzed using Slidebook software (Intelligent Imaging Innovations). .. For immunofluorescence staining of thymus, freshly frozen thymi were cryosectioned and stained as outlined above using biotinylated UEA-1 lectin (Vector Labs). .. Sections were washed in PBS before incubation with AF568-conjugated streptavidin (ThermoFisher) and 10 nM DAPI.

    Incubation:

    Article Title: Nfkb2 variants reveal a p100-degradation threshold that defines autoimmune susceptibility
    Article Snippet: Samples were cut into 8-μm cryosections using a Micron HM550 Cryostat (Thermo Fisher Scientific), air dried, fixed in ice-cold acetone for 1 min, air dried, and blocked with 5% (vol/vol) goat serum in PBS with 0.5% Tween-20 (vol/vol) for 30 min at room temperature. .. Sections were incubated for 30 min at room temperature with primary antibodies, including anti-K5 (catalog no. 905504; BioLegend), anti-K14 (catalog no. 90530; BioLegend), biotinylated UEA-1 lectin (catalog no. B-1065; Vector Laboratories), biotinylated anti-mouse AIRE (clone 5H12; Walter and Eliza Hall Institute), and anti-K8 (clone TROMA-I; Developmental Studies Hybridoma Bank). .. Following three 5-min washes in PBS, sections were incubated with secondary reagents including Donkey anti-rabbit IgG Alexa Fluor 555 (catalog no. A-31572; Invitrogen); Goat anti-rat IgG Alexa Fluor 555 (catalog no. A-21434; Invitrogen); Donkey anti-rabbit IgG Alexa Fluor 647 (catalog no. A-21244; Invitrogen), and Alexa Fluor 488 conjugated streptavidin (catalog no. S32354; Invitrogen) for 30 min at room temperature.



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